کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8270345 | 1534971 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DCFH-DAVCHRT PCRmRNA gene expressionδ-ALA-DNRF-2HspDTNBRT-PCRVCDGSTmRNA4-vinylcyclohexene - 4-وینیل سیکلوهاکسنmessenger RNA - RNA messengerROS - ROSAntioxidants - آنتی اکسیدانAChE - آهیAcetylcholinesterase - استیل کولین استرازmEH - بلهOxidative stress - تنش اکسیداتیو4-Vinylcyclohexene diepoxide - دیاپکسید 4-وینیل سیکلوکسنSOD - سدNeurotoxicity - سمیت عصبیSuperoxide dismutase - سوکسوکس دیسموتازnuclear factor erythroid 2-related factor 2 - فاکتور هسته ای عامل erythroid 2 مرتبط 2Microsomal epoxide hydrolase - هیدرولاز اپوکسی میکروسیمالreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسHeat shock protein - پروتئین شوک حرارتReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
4-Vinylcyclohexene (VCH) is a dimer of 1,3-butadiene produced as a by-product of pesticides, plastic, rubber, flame retardants, and tire production. Although, several studies have reported the ovotoxicity of VCH, information on a possible involvement of oxidative stress in the toxicity of this occupational chemical is scarce. Hence, this study was carried out to investigate further possible mechanisms of toxicity of VCH with a specific emphasis on oxidative stress using a Drosophila melanogaster model. D. melanogaster (both genders) of 1 to 3 days old were exposed to different concentrations of VCH (10 µM-1 mM) in the diet for 5 days. Subsequently, the survival and negative geotaxis assays and the quantification of reactive oxygen species (ROS) generation were determined. In addition, we evaluated RT-PCR expressions of selected oxidative stress and antioxidant mRNA genes (HSP27, 70, and 83, SOD, Nrf-2, MAPK2, and catalase). Furthermore, catalase, glutathione-S-transferase (GST), delta aminolevulinic acid dehydratase (δ-ALA-D), and acetylcholinesterase (AChE) activities were determined. VCH exposure impaired negative geotaxic behavior and induced the mRNA of SOD, Nrf-2, and MAPK2 genes expressions. There were increases in catalase and ROS production, as well as inhibitions of GST, δ-ALA-D, and AChE activities (P<0.05). Our results suggest that the VCH mechanism of toxicity is associated with oxidative damage, as evidenced by the alteration in the oxidative stress-antioxidant balance, and possible neurotoxic consequences due to decreased AChE activity, and impairments in negative geotaxic behavior. Thus, we conclude that D. melanogaster is a useful model for investigating the toxicity of VCH exposure, and here, we have provided further insights on the mechanism of VCH-induced toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 71, June 2014, Pages 99-108
Journal: Free Radical Biology and Medicine - Volume 71, June 2014, Pages 99-108
نویسندگان
Amos Olalekan Abolaji, Jean Paul Kamdem, Thiago Henrique Lugokenski, Thallita Kalar Nascimento, Emily Pansera Waczuk, Ebenezer Olatunde Farombi, Ãlgion Lúcio da Silva Loreto, João Batista Teixeira Rocha,