Measurement of F2- isoprostanes and isofurans using gas chromatography-mass spectrometry

► F2-Isoprostanes (IsoPs) are products of free radical-catalyzed lipid peroxidation. ► Isofurans (IsoFs) are formed instead of F2-IsoPs when oxygen tension is elevated. ► GC/MS is used to measure F2-IsoPs and IsoFs as biomarkers of oxidative stress. ► F2-IsoPs and IsoFs are increased in a variety of human conditions and diseases.