کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8271774 | 1534990 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interrupted reperfusion reduces the activation of NADPH oxidase after cerebral I/R injury
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CPURac1CA1I/R - I / RO2•− - O2 • -ROS - ROSIschemia/reperfusion injury - آسیب شناسی ایسکمی / رپرفیوژنcornu Ammonis 1 - آمون شاخ 1superoxide anion - آنیون سوپر اکسیدNADPH oxidase - اکسیداز NADPH ischemia/reperfusion - ایسکمی / رپرفیوژنFree radicals - رادیکال آزادsensorimotor cortex - قشر حسگر حرکتیHydroxide - هیدروکسیدCaudate putamen - پاتامن CaudateReactive oxygen species - گونههای فعال اکسیژن•OH - • OH
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Interrupted reperfusion reduces ischemia/reperfusion (I/R) injury. This study was designed to determine whether NADPH oxidase participates in the neural protection against global I/R injury after interrupted reperfusion. Mice were randomly divided into five groups: sham (sham-operated), I/R (20-min global I/R), RR (I/RÂ +Â interrupted reperfusion), Apo (I/RÂ +Â apocynin administration), and RRÂ +Â Apo. Behavioral tests (pole test, beam walking, and Morris water maze) and Nissl staining were undertaken in all five groups; superoxide levels, expression of gp91phox and p47phox, p47phox translocation, and Rac1 activation were measured in the sham, I/R, and RR groups. The motor coordination, bradykinesia, and spatial learning and memory, as well as the neuron survival rates, were better in the RR, Apo, and RRÂ +Â Apo groups than in the I/R group. The NADPH oxidase-dependent superoxide levels, p47phox and gp91phox expression, p47phox translocation, and Rac1 activation were lower in the RR group than in the I/R group. In conclusion, the neural protective effect of interrupted reperfusion is at least partly mediated by decreasing the expression and assembly of NADPH oxidase and the levels of NADPH oxidase-derived superoxide. The most striking reduction Rac1-GTP in the RR group suggests that interrupted reperfusion also acts on the activation of assembled NADPH oxidase by reducing the availability of Rac1-GTP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 50, Issue 12, 15 June 2011, Pages 1780-1786
Journal: Free Radical Biology and Medicine - Volume 50, Issue 12, 15 June 2011, Pages 1780-1786
نویسندگان
Jia Shen, Xiao-Yin Bai, Yuan Qin, Wei-Wei Jin, Jing-Yin Zhou, Ji-Ping Zhou, Ying-Gang Yan, Qiong Wang, Iain C. Bruce, Jiang-Hua Chen, Qiang Xia,