کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8284780 | 1535601 | 2016 | 20 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of calorie reduction on DNA repair capacity of human peripheral blood mononuclear cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSFACShost cell reactivation assayPBMCGFPNER - DOWNEDTA - اتیلن دی آمین تترا استیک اسید ethylene diamine tetraacetic acid - اتیلن دیامین تتراستیک اسیدUltraviolet - اشعه فرابنفشnucleotide excision repair - تعمیر مجدد نوکلئوتیدیstandard error of the mean - خطای استاندارد میانگینfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیHuman peripheral blood mononuclear cells - سلول های تک سلولی خون محیطی انسانDNA repair capacity - ظرفیت تعمیر DNAPhosphate buffered saline - فسفات بافر شورSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Caloric restrictive feeding prolongs the lifespan of a variety of model organisms like rodents and invertebrates. It has been shown that caloric restriction reduces age-related as well as overall-mortality, reduces oxidative stress and influences DNA repair ability positively. There are numerous studies underlining this, but fewer studies involving humans exist. To contribute to a better understanding of the correlation of calorie reduction and DNA repair in humans, we adapted the host cell reactivation assay to an application with human peripheral blood mononuclear cells. Furthermore, we used this reliable and reproducible assay to research the influence of a special kind of calorie reduction, namely F. X. Mayr therapy, on DNA repair capacity. We found a positive effect in all persons with low pre-existing DNA repair capacity. In individuals with normal pre-existing DNA repair capacity, no effect on DNA repair capacity was detectable. Decline of DNA repair, accumulation of oxidative DNA damages, mitochondrial dysfunction, telomere shortening as well as caloric intake are widely thought to contribute to aging. With regard to that, our results can be considered as a strong indication that calorie reduction may support DNA repair processes and thus contribute to a healthier aging.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volume 154, March 2016, Pages 24-29
Journal: Mechanisms of Ageing and Development - Volume 154, March 2016, Pages 24-29
نویسندگان
Katja Matt, Katharina Burger, Daniel Gebhard, Jörg Bergemann,