کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8287526 | 1535856 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression patterns of claudin-5 and its related signals during luteal regression in pseudopregnant rats: The enhanced effect of additional PGF treatment
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کلمات کلیدی
p-ERKphosphorylated ERK1/2STAT3PGFP450sccPDK1p-STAT3PKCPGF2αp383β-HSDECLmTORC2Cytochrome P450sccp-p38MAPK - MAPKp-Akt - P-AKTp38 MAPK - P38 MAPKTJs - TJStight junctions - اتصالات محکمcorpus luteum - جسم زردStar - ستارهphosphorylated Akt - فسفروئید آکتphosphorylated STAT3 - فسفریک شده STAT3Signal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیRats - موش صحراییMammalian target of rapamycin complex 2 - هدف پستانداران رپامایسین 2phosphoinositide-dependent kinase 1 - وابسته به فسفوئینوزیتید کیناز 1Steroidogenic acute regulatory protein - پروتئین حاکم استروئیدوژنیک حادProtein kinase C - پروتئین کیناز سیProgesterone - پروژسترونenhanced chemoluminescence - چمولومینسانس پیشرفتهClaudin-5 - کلودین-5
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression patterns of claudin-5 and its related signals during luteal regression in pseudopregnant rats: The enhanced effect of additional PGF treatment Expression patterns of claudin-5 and its related signals during luteal regression in pseudopregnant rats: The enhanced effect of additional PGF treatment](/preview/png/8287526.png)
چکیده انگلیسی
To study the expression patterns of claudin-5 and its related signals during luteal regression in rats, a sequential PMSG/hCG treatment paradigm was used to obtain a single, well-defined generation of corpus luteum (CL). A total of 35 rats were treated with one PGF or two PGF at an interval of 24â¯h from day 7 of pseudopregnancy to induce CL regression. Serum and ovaries were collected at 0, 2, 4, 8 or 24â¯h after one PGF injection (1 PGF), 2 or 24â¯h after two PGF injections (2 PGF). The serum progesterone level was detected by RIA; the ovarian expression of claudin-5, the phosphorylations of STAT3 (p-STAT3), Akt (p-Akt), ERK1/2 (p-ERK) and p38 MAPK (p-p38) were detected by western blot, real-time PCR and IHC. Results showed that serum progesterone (P4) decreased after PGF treatment. Claudin-5 mRNA decreased at 4â¯h and 8â¯h after 1 PGF and 2â¯h after 2 PGF, and claudin-5 protein decreased at 4â¯h after 1 PGF. p-STAT3 increased at 4â¯h after 1 PGF and 2â¯h after 2 PGF. p-ERK increased at 2â¯h after 2 PGF. The level of p-Akt decreased at 4â¯h after 1 PGF. PGF treatment did not alter the phosphorylation of p38 MAPK at any time points in this study. IHC results revealed that claudin-5 was expressed in the nuclei and cytoplasm of steroidogenic cells and in the vessels, while PGF induced-p-STAT3 was expressed uniformly in the cytoplasm of luteal steroidogenic cells. In conclusion, PGF treatment decreased the expression of claudin-5 and the additional PGF treatment enhanced the decrease in claudin-5 mRNA expression and the increases in ERK1/2 and STAT3 phosphorylation in the corpus luteum of pseudopregnant rats, which will contribute new information to the further study of molecular mechanism of luteal regression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 120, Issue 3, April 2018, Pages 221-227
Journal: Acta Histochemica - Volume 120, Issue 3, April 2018, Pages 221-227
نویسندگان
Lina Qi, Jingle Jiang, Pengjin Jin, Meiqian Kuang, Quanwei Wei, Fangxiong Shi, Dagan Mao,