Anti-EGFR biparatopic-SEED antibody has enhanced combination-activity in a single molecule

► Combinations of anti-EGFR antibodies are reported to result in enhanced activity. ► Anti-EGFR biparatopic-SEED antibody was created with two different binding domains. ► Anti-EGFR biparatopic-SEED had enhanced combination-activity in a single molecule. ► Cross-linking two different epitopes is important for enhanced combination-activity.