Characterisation of the N′1 isoform of the cyclic AMP-dependent protein kinase (PK-A) catalytic subunit in the nematode, Caenorhabditis elegans

► N′1-isoforms of Caenorhabditis elegans PK-A catalytic subunits are N-myristoylatable. ► The presence of myristoyl-glycine has been confirmed by MALDI-TOF MS. ► Protein with C. elegans N-terminus and murine C-terminus has been prepared. ► Myristoylation has little effect on the catalytic properties of the chimera. ► Myristoylated chimera has greater apolar targeting than murine protein.