Nanosecond pulsed electric fields activate MAPK pathways in human cells

► Nanosecond pulsed electric field (nsPEF) is known as a new tool for cancer therapy. ► Human MAPKs are phosphorylated in a time-dependent manner after nsPEF application. ► Upstream and downstream events in the MAPK pathways are activated by nsPEFs. ► Our study provides a critical mechanistic clue to nsPEF actions in human cells.