Characterization of the regulatory subunit of Yarrowia lipolytica cAMP-dependent protein kinase. Evidences of a monomeric protein

► Characterization of the regulatory subunit of Yarrowia lipolytica PKA. ► Cloning and heterologous expression of an active Y. lipolytica regulatory subunit PKA holoenzyme is a dimeric protein of type RC. ► Regulatory subunits can not homodimerize due to differences in the N-terminus. ►Y. lipolytica R subunit is phosphorylated in vivo at S124, thus belonging to type II.