Crystal structure and immunogenicity of the class C acid phosphatase from Pasteurella multocida

► First structure of a class C acid phosphatase in the metal-free state. ► Unusual crystal packing involving the polyhistidine affinity tag mimicking the substrate. ► Production of anti-phosphatase antibodies. ► Western blot detection of wild-type Pasteurella multocida class C phosphatase in clinical strains