Amphipathic C-terminal region of Escherichia coli NADH dehydrogenase-2 mediates membrane localization

► NDH-2 is a peripheral membrane protein, interacting with membrane by amphipathic regions. ► C-terminal amphipathic Arg390-Ala406 helix plays a key role in NDH-2/membrane binding. ► Elimination of NDH-2 C-terminal region leads to an active and water soluble protein. ► The achievement of a water soluble enzyme serves as a major tool for further studies.