Purification, characterization and inhibition of sterol C24-methyltransferase from Candida albicans

► Wild-type sterol C24-methyltransferase for the first time from any source - Candida albicans - has been purified and characterized. ► Turnover number of CaSMT established to be 0.01s−1. ► Sterol biosynthesis inhibitors that target sterol C24-methyltransferase have been evaluated in vitro and in vivo. ► Ergosterol but not cholesterol or sitosterol shown to be an allosteric modulator of sterol C24-methyltransferase activity.