کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8291993 | 1536478 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Suppression of metabolic defects of yeast isocitrate dehydrogenase and aconitase mutants by loss of citrate synthase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Yeast mutants lacking mitochondrial NAD+-specific isocitrate dehydrogenase (idhÎ) or aconitase (aco1Î) were found to share several growth phenotypes as well as patterns of specific protein expression that differed from the parental strain. These shared properties of idhÎ and aco1Î strains were eliminated or moderated by co-disruption of the CIT1 gene encoding mitochondrial citrate synthase. Gas chromatography/mass spectrometry analyses indicated a particularly dramatic increase in cellular citrate levels in idhÎ and aco1Î strains, whereas citrate levels were substantially lower in idhÎcit1Î and aco1Îcit1Î strains. Exogenous addition of citrate to parental strain cultures partially recapitulated effects of high endogenous levels of citrate in idhÎ and aco1Î strains. Finally, effects of elevated cellular citrate in idhÎ and aco1Î mutant strains were partially alleviated by addition of iron or by an increase in pH of the growth medium, suggesting that detrimental effects of citrate are due to elevated levels of the ionized form of this metabolite.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 474, Issue 1, 1 June 2008, Pages 205-212
Journal: Archives of Biochemistry and Biophysics - Volume 474, Issue 1, 1 June 2008, Pages 205-212
نویسندگان
An-Ping Lin, Kevin W. Hakala, Susan T. Weintraub, Lee McAlister-Henn,