کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8298589 | 1537034 | 2018 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultrafast multi-pulse transient absorption spectroscopy of fucoxanthin chlorophyll a protein from Phaeodactylum tricornutum
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have applied femtosecond transient absorption spectroscopy in pump-probe and pump-dump-probe regimes to study energy transfer between fucoxanthin and Chl a in fucoxanthin-Chl a complex from the pennate diatom Phaeodactylum tricornutum. Experiments were carried out at room temperature and 77â¯K to reveal temperature dependence of energy transfer. At both temperatures, the ultrafast (<100â¯fs) energy transfer channel from the fucoxanthin S2 state is active and is complemented by the second pathway via the combined S1/ICT state. The S1/ICT-Chl a pathway has two channels, the fast one characterized by sub-picosecond energy transfer, and slow having time constants of 4.5â¯ps at room temperature and 6.6â¯ps at 77â¯K. The overall energy transfer via the S1/ICT is faster at 77â¯K, because the fast component gains amplitude upon lowering the temperature. The pump-dump-probe regime, with the dump pulse centered in the spectral region of ICT stimulated emission at 950â¯nm and applied at 2â¯ps after excitation, proved that the S1 and ICT states of fucoxanthin in FCP are individual, yet coupled entities. Analysis of the pump-dump-probe data suggested that the main energy donor in the slow S1/ICT-Chl a route is the S1 part of the S1/ICT potential surface.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 5, May 2018, Pages 357-365
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 5, May 2018, Pages 357-365
نویسندگان
Robert G. West, David BÃna, Marcel Fuciman, Valentyna Kuznetsova, Radek LitvÃn, TomáÅ¡ PolÃvka,