Effect of juxtamembrane tryptophans on the immersion depth of Synaptobrevin, an integral vesicle membrane protein

► We examine the positioning of neuronal-Synaptobrevin within micelles. ► Mutants with zero and two Trp residues are compared to wild-type (one Trp). ► Mutations of juxtamembrane tryptophan residues affect membrane positioning. ► Decreasing Trp content results in deeper immersion of the peptide in the micelle ► Increasing Trp content dampens peptide dynamics near the water-micelle interface.