Oligomeric state study of prokaryotic rhomboid proteases

► Prokaryotic rhomboids form oligomers when solubilized in detergent. ► Crosslinking and gel filtration of hiGlpG indicates a dimeric species. ► Co-IP suggests that dimers of hiGlpG form in the lipid bilayer. ► Membrane domain is the minimal unit for rhomboid oligomerization.