Multi-Tox: Application of the ToxR-transcriptional reporter assay to the study of multi-pass protein transmembrane domain oligomerization

► Multi-Tox studies multi-pass TMD oligomerization by ToxR reporter assay. ► Multi-Tox was applied to study homo- and hetero-oligomerizations. ► LMP-1 oligomerization is driven by a buried aspartic acid in helix five. ► K94 was identified as playing a key role in DAGK trimerization.