Nonsense-mediated mRNA decay - Mechanisms of substrate mRNA recognition and degradation in mammalian cells

► Translation termination distant from PABP triggers NMD. ► EJCs are enhancers of NMD and differentially deposited onto spliced mRNA. ► UPF1 ATPase and RNA helicase is tightly regulated ► Phosphorylation of UPF1 is crucial for NMD but appears to not affect UPF1 ATPase/helicase activities. ► SMG5, SMG6, SMG7 and PNRC2 appear to promote mRNA decay through different routes.