کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8300707 | 1537471 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolic cofactors NAD(P)H and FAD as potential indicators of cancer cell response to chemotherapy with paclitaxel
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کلمات کلیدی
IC50reduced nicotinamide adenine dinucleotide (phosphate)TCAFBSFLIM - FL منNAD(P)H - NAD (P) HROS - ROSFAD - بدfetal bovine serum - سرم جنین گاوCancer cell - سلول سرطانیChemotherapy - شیمیدرمانیfluorescence lifetime imaging microscopy - میکروسکوپ تصویربرداری طول عمر فلورسنتhalf-inhibitory concentration - نیمه مهار کننده غلظتEarly response - پاسخ اولیهPaclitaxel - پاکلی تاکسلPropidium iodide - پروتئین یدیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Metabolic cofactors NAD(P)H and FAD as potential indicators of cancer cell response to chemotherapy with paclitaxel Metabolic cofactors NAD(P)H and FAD as potential indicators of cancer cell response to chemotherapy with paclitaxel](/preview/png/8300707.png)
چکیده انگلیسی
Paclitaxel, a widely used antimicrotubular agent, predominantly eliminates rapidly proliferating cancer cells, while slowly proliferating and quiescent cells can survive the treatment, which is one of the main reasons for tumor recurrence and non-responsiveness to the drug. To improve the efficacy of chemotherapy, biomarkers need to be developed to enable monitoring of tumor responses. In this study we considered the auto-fluorescent metabolic cofactors NAD(P)H and FAD as possible indicators of cancer cell response to therapy with paclitaxel. It was found that, among the tested parameters (the fluorescence intensity-based redox ratio FAD/NAD(P)H, and the fluorescence lifetimes of NAD(P)H and FAD), the fluorescence lifetime of NAD(P)H is the most sensitive in tracking the drug response, and is capable of indicating heterogeneous cellular responses both in cell monolayers and in multicellular tumor spheroids. We observed that metabolic reorganization to a more oxidative state preceded the morphological manifestation of cell death and developed faster in cells that were more responsive to the drug. Our results suggest that noninvasive, label-free monitoring of the drug-induced metabolic changes by noting the NAD(P)H fluorescence lifetime is a valuable approach to characterize the responses of cancer cells to anti-cancer treatments and, therefore, to predict the effectiveness of chemotherapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1862, Issue 8, August 2018, Pages 1693-1700
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1862, Issue 8, August 2018, Pages 1693-1700
نویسندگان
Maria M. Lukina, Varvara V. Dudenkova, Nadezhda I. Ignatova, Irina N. Druzhkova, Lyubov' E. Shimolina, Elena V. Zagaynova, Marina V. Shirmanova,