Releasing iron from ferritin protein nanocage by reductive method: The role of electron transfer mediator

PMS and PYO releases more iron from ferritin nanocages than FMN. Ferritin protein nanocages synthesize ferrihydrite mineral core in their central cavity. Pyocyanin (PYO), a microbial virulence factor and PMS mobilized more iron as compared to FMN, owing to their better ET relay ability (faster rate of NADH oxidation, dissolved O2 consumption, and mineral core reduction). Inset: simultaneous monitoring of dissolved O2 consumption (left Y-axis) and % of iron released (right Y-axis) in the form of Fe(II)-ferrozine complex from frog M ferritin during the reductive pathway.217