کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8302150 | 1537721 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CTGF/CCN2 exerts profibrotic action in myoblasts via the up-regulation of sphingosine kinase-1/S1P3 signaling axis: Implications in the action mechanism of TGFβ
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کلمات کلیدی
S1PDMEMFCSα-SMADPBSTGFβCTGFECLBSA - BSADulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccoshort interfering RNA - RNA تداخل کوتاهSDS–PAGE - SDS-PAGEsiRNA - siRNAbovine serum albumin - آلبومین سرم گاوSphingosine kinase - اسپینوزین کینازsphingosine 1-phosphate - اسپینگزین 1-فسفاتsodium dodecyl sulfate–polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدα-smooth muscle actin - اکتین عضله آلفا صافenhanced chemiluminescence - بهبود شیمیایی لومنTransforming Growth Factor Beta - تبدیل بتا فاکتور رشدscrambled - تقلا کردfetal calf serum - سرم گوساله جنینConnective tissue growth factor - فاکتور رشد بافت همبندMyoblasts - میوبلاست هاPropidium iodide - پروتئین یدیدSCR - یکسوساز کنترلشده با سیلیکون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The matricellular protein connective tissue growth factor (CTGF/CCN2) is recognized as key player in the onset of fibrosis in various tissues, including skeletal muscle. In many circumstances, CTGF has been shown to be induced by transforming growth factor beta (TGFβ) and accounting, at least in part, for its biological action. In this study it was verified that in cultured myoblasts CTGF/CCN2 causes their transdifferentiation into myofibroblasts by up-regulating the expression of fibrosis marker proteins α-smooth muscle actin and transgelin. Interestingly, it was also found that the profibrotic effect exerted by CTGF/CCN2 was mediated by the sphingosine kinase (SK)-1/S1P3 signaling axis specifically induced by the treatment with the profibrotic cue. Following CTGF/CCN2-induced up-regulation, S1P3 became the S1P receptor subtype expressed at the highest degree, at least at mRNA level, and was thus capable of readdressing the sphingosine 1-phosphate signaling towards fibrosis rather than myogenic differentiation. Another interesting finding is that CTGF/CCN2 silencing prevented the TGFβ-dependent up-regulation of SK1/S1P3 signaling axis and strongly reduced the profibrotic effect exerted by TGFβ, pointing at a crucial role of endogenous CTGF/CCN2 generated following TGFβ challenge in the transmission of at least part of its profibrotic effect. These results provide new insights into the molecular mechanism by which CTGF/CCN2 drives its biological action and strengthen the concept that SK1/S1P3 axis plays a critical role in the onset of fibrotic cell phenotype.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1851, Issue 2, February 2015, Pages 194-202
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1851, Issue 2, February 2015, Pages 194-202
نویسندگان
Gennaro Bruno, Francesca Cencetti, Irene Pertici, Lukasz Japtok, Caterina Bernacchioni, Chiara Donati, Paola Bruni,