Spatially resolved investigation of the oil composition in single intact hyphae of Mortierella spp. with micro-Raman spectroscopy

► Lipid vesicles inside intact hyphae of Mortierella are investigated. ► The degree of unsaturation of the oil is determined with Raman spectroscopy. ► The vesicle composition is highly variable in the first 600 μm of the hyphal tips. ► Lipid composition is homogeneous in all older hyphal parts throughout the mycelium.