The glycolipid transfer protein (GLTP) domain of phosphoinositol 4-phosphate adaptor protein-2 (FAPP2): Structure drives preference for simple neutral glycosphingolipids

► FAPP2 enables glycosphingolipid synthesis in the Golgi by transfer of glucosylceramide. ► FAPP2 contains a modified GLTP-fold that can also transfer other neutral glycosphingolipids. ► Glycolipid selectivity of FAPP2-GLTP is more focused than human GLTP. ► Glycolipid compartmentation appears to mute evolutionary selection pressure and divergence.