کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8304442 1538409 2016 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Strategies for detection and quantification of cysteine cathepsins-evolution from bench to bedside
ترجمه فارسی عنوان
استراتژی برای تشخیص و اندازه گیری کیتپسین های سستی-تکامل از نیمکت به بالشتک
کلمات کلیدی
پروتئیناز سیتین، پروب وابسته به وابستگی، پروب مبتنی بر فعالیت، کاتهپسین، پروتئولیز، بستر خرد شده،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
The cysteine cathepsins are a family of closely related thiol proteases, normally found in the endosomal and lysosomal compartments of cells. A growing body of evidence has clearly linked the dysregulated activity of these proteases with many diseases and pathological conditions, offering therapeutic, prognostic and diagnostic potential. However, these proteases are synthesised as inactive precursors and once activated, are controlled by factors such as pH and presence of endogenous inhibitors, meaning that overall protein and activity levels do not necessarily correlate. In order to fully appreciate the role and potential of these proteases, tools are required that can detect and quantify overall cathepsin activity. Two main strategies have evolved; synthetic substrates and protease-labelling with affinity-binding probes (or activity-based probes). This review examines recent innovations in these approaches as the field moves towards developing tools that could ultimately be used in patients for diagnostic or prognostic applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 122, March 2016, Pages 48-61
نویسندگان
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