کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8304769 1538413 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG
چکیده انگلیسی
Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 118, November 2015, Pages 116-122
نویسندگان
, , , , ,