کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8309877 | 1538637 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The association of circulating microRNA-30c with atherogenic lipoprotein subfractions and composition
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
qPCRCETPmRNAMIRMTPmessenger RNA - RNA messengerreverse transcription - رونویسی معکوسMicroRNA - میکرو RNA quantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استmicrosomal triglyceride transfer protein - پروتئین انتقال تری گلیسرید میکروسمالیquantification cycle - چرخه محاسبه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The association of circulating microRNA-30c with atherogenic lipoprotein subfractions and composition The association of circulating microRNA-30c with atherogenic lipoprotein subfractions and composition](/preview/png/8309877.png)
چکیده انگلیسی
Circulating miR-30c has been linked to various aspects of cholesterol homeostasis. The aim of this study was to determine the association of circulating miR-30c with the atherogenic lipoprotein subfractions. Samples from subjects who were given placebo (n = 22) in a randomised, double-blind crossover study were used. Subjects were divided into non-atherogenic lipoprotein phenotype (Non-ALP; n = 12; triglycerides < 2.0 mmol/L) and atherogenic lipoprotein phenotype (ALP; n = 10; triglycerides â¥Â 2.0 mmol/L) groups. All lipid and lipoprotein measurements, RNA extraction and reverse transcription-quantitative real-time polymerase chain reaction were undertaken using standard procedures. Subjects with ALP weighed significantly more than their non-ALP counterparts (p = 0.023). In the non-ALP group there was significant correlation between miR-30c and components within VLDL1, namely triglyceride which showed a negative association (p = 0.035) whereas phospholipids and cholesterol-ester were both positively correlated (p = 0.025 and 0.014, respectively). In contrast, in the ALP group there was a significant correlation between the expression of miR-30c and components within VLDL2, namely triglyceride, which was positively associated (p = 0.013). This study reveals specificity with regards to the effect of miR-30c on VLDL subfractions based on the individual's lipoprotein phenotype and implicates roles for microsomal-triglyceride transfer-protein and cholesteryl-ester-transfer-protein in LDL and VLDL metabolism, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 462, 1 November 2016, Pages 135-139
Journal: Clinica Chimica Acta - Volume 462, 1 November 2016, Pages 135-139
نویسندگان
Jarlath Eastwood, Muriel J Caslake, Ravinder Sodi,