کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8313519 | 1538677 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A linear relationship between the ex-vivo sodium mediated expression of two sodium regulatory pathways as a surrogate marker of salt sensitivity of blood pressure in exfoliated human renal proximal tubule cells: The virtual renal biopsy
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A linear relationship between the ex-vivo sodium mediated expression of two sodium regulatory pathways as a surrogate marker of salt sensitivity of blood pressure in exfoliated human renal proximal tubule cells: The virtual renal biopsy A linear relationship between the ex-vivo sodium mediated expression of two sodium regulatory pathways as a surrogate marker of salt sensitivity of blood pressure in exfoliated human renal proximal tubule cells: The virtual renal biopsy](/preview/png/8313519.png)
چکیده انگلیسی
BackgroundSalt sensitivity (SS) of blood pressure (BP) affects 25% of adults, shares comorbidity with hypertension, and has no convenient diagnostic test. We tested the hypothesis that urine-derived exfoliated renal proximal tubule cells (RPTCs) could diagnose the degree of an individual's SS of BP.MethodsSubjects were selected who had their SS of BP determined 5 y prior to this study (salt-sensitive: â¥Â 7 mm Hg increase in mean arterial pressure (MAP) following transition from a random weekly diet of low (10 mmol/day) to high (300 mmol/day) sodium (Na+) intake, N = 4; inverse salt-sensitive (ISS): â¥Â 7 mm Hg increase in MAP transitioning from a high to low Na+ diet, N = 3, and salt-resistant (SR): < 7 mm Hg change in MAP transitioned on either diet, N = 5). RPTC responses to 2 independent Na+ transport pathways were measured.ResultsThere was a negative correlation between the degree of SS and dopamine-1 receptor (D1R) plasma membrane recruitment (y = â 0.0107x + 0.68 relative fluorescent units (RFU), R2 = 0.88, N = 12, P < 0.0001) and angiotensin II-stimulated intracellular Ca++ (y = â 0.0016x + 0.0336, R2 = 0.7112, P < 0.001, N = 10) concentration over baseline.ConclusionsIsolating RPTCs from urine provides a personalized cell-based diagnostic test of SS index that offers advantages over a 2-week controlled diet with respect to cost and patient compliance. Furthermore, the linear relationship between the change in MAP and response to 2 Na+ regulatory pathways suggests that an individual's RPTC response to intracellular Na+ is personalized and predictive.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 421, 5 June 2013, Pages 236-242
Journal: Clinica Chimica Acta - Volume 421, 5 June 2013, Pages 236-242
نویسندگان
John J. Gildea, Dylan T. Lahiff, Robert E. Van Sciver, Ryan S. Weiss, Neema Shah, Helen E. McGrath, Cynthia D. Schoeffel, Pedro A. Jose, Robert M. Carey, Robin A. Felder,