Detection of autoantibodies against platelet glycoproteins in patients with immune thrombocytopenic purpura by flow cytometric immunobead array

► Immune thrombocytopenic purpura (ITP) is a bleeding disorder associated with platelet autoantibodies. ► We developed a flow cytometric immunobead array (FCIA) assay using a panel of antibodies against platelet glycoproteins. ► The FCIA assay detected high levels of platelet autoantibodies in ITP patients. ► The assay significantly improved the sensitivity and accuracy in ITP diagnosis.