Development of a novel immunoassay for the measurement of type II collagen neoepitope generated by collagenase cleavage

► We developed a novel sandwich ELISA for the type II collagen neoepitope (CIINE). ► The ELISA determined CIINE concentration regardless of hydroxylation of Pro771. ► The ELISA can determine CIINE concentration in human urine accurately. ► Urinary CIINE level of OA patients was higher than that of healthy controls.