Lysosomal storage disorder 4Â +Â 1 multiplex assay for newborn screening using tandem mass spectrometry: Application to a small-scale population study for five lysosomal storage disorders

► We performed a small-scale population study for five lysosomal storage disorders. ► The assay shows good separation between normal and diseased controls. ► We simplified the assay by eliminating first the pre-elution extraction step. ► We further simplified by reducing buffers needed from five to two.