A novel and precise method for simultaneous measurement of serum HDL and LDL subfractions and lipoprotein (a) cholesterol by ultracentrifugation and high-performance liquid chromatography

► Serum Lp(a) is dissociated into apo(a) and apo(a) depleted Lp(a-). ► Lp(a-) shows a distinctive density distribution from native Lp(a). ► Separation of lipoproteins by ultracentrifugation is not contaminated with Lp(a). ► HPLC is used for measurement of lipoprotein subfraction and Lp(a) cholesterol. ► This method is a potentially useful technique for lipoprotein profiling.