Standardization non-invasive fetal RHD and SRY determination into clinical routine using a new multiplex RT-PCR assay for fetal cell-free DNA in pregnant women plasma: Results in clinical benefits and cost saving

► New method to evaluate fetal RHD in the pregnant plasma using multiplex real time PCR. ► The method allows the automatization of non-invasive prenatal diagnosis of fetal RHD. ► The assay is 100% sensitive to detect RHD positive fetuses since week 10 of pregnancy.