Rapid identification of CYP2C8 polymorphisms by high resolution melting analysis

► Genomic DNA was extracted from peripheral blood samples from 95 normal Taiwanese. ► A high resolution melting analysis was used to characterize the CYP2C8 polymorphism. ► All results were confirmed by direct DNA sequencing. ► Five new single nucleotide polymorphisms were found in exon 8 and exon 9. ► HRM analysis is a cost-effective screening method for gene mutation.