Differential detection of KRAS mutations in codons 12 and 13 with a modified loop-hybrid (LH) mobility shift assay using an insert-type LH-generator

► LH-MSA is a simple method for detection of point-mutations. ► LH-MSA detected 12 mutations of KRAS in codons 12 and 13. ► The insert type LH-probe provided a way to detect these 12 mutations. ► Our method differentiated all KRAS mutations detected in colorectal tumor DNA. ► LH-MSA detected KRAS mutations in colorectal tumor DNA with 5% mutant sensitivity.