Rapid simultaneous determination of voriconazole and its N-oxide in human plasma using an isocratic high-performance liquid chromatography method and its clinical application

► We simultaneously determined voriconazole and its N-oxide in human plasma. ► The samples with internal standard were injected into HPLC system with UV detector. ► The determination finished within 4 min and was accurate and reproducible. ► The established method was successfully applied to clinical samples.