کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8319161 | 1539292 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short-term treatment of adult male zebrafish (Danio Rerio) with 17α-ethinyl estradiol affects the transcription of genes involved in development and male sex differentiation
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کلمات کلیدی
ER-αsex determining region Y18S RNAcyp19aDMY17α-ethinyl estradiolSOX9ADMRT1ER-βEE2qPCREDCVitellogeninSTP - PTSEndocrine disruptors - اختلالهای غدد درون ریزEstrogen - استروژنAMH - اوBiomarker - بیومارکرSewage treatment plant - تصفیه خانه فاضلابSex differentiation - تمایز جنسیتیGene regulation - تنظیم ژنSry - سوریendocrine disrupting chemical - شیمیایی خرابکار غدد درون ریزAnti-Müllerian hormone - هورمون Anti-Mellerianquantitative real time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیvtg - ویتگGonads - گنادهاZebrafish - گورخرماهیAndrogen Receptor - گیرنده آندروژنیEstrogen receptor alpha - گیرنده استروژن آلفاEstrogen receptor beta - گیرنده استروژن بتا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Short-term treatment of adult male zebrafish (Danio Rerio) with 17α-ethinyl estradiol affects the transcription of genes involved in development and male sex differentiation Short-term treatment of adult male zebrafish (Danio Rerio) with 17α-ethinyl estradiol affects the transcription of genes involved in development and male sex differentiation](/preview/png/8319161.png)
چکیده انگلیسی
The synthetic estrogen 17α-ethinyl estradiol (EE2) disturbs reproduction and causes gonadal malformation in fish. Effects on the transcription of genes involved in gonad development and function that could serve as sensitive biomarkers of reproductive effects in the field is, however, not well known. We have studied mRNA expression in testes and liver of adult zebrafish (Danio rerio) males treated with 0, 5 or 25 ng/L EE2for 14 days. qPCR analysis showed that the mRNA expression of four genes linked to zebrafish male sex determination and differentiation, Anti-Mullerian Hormone, Double sex and mab-related protein, Sry-related HMG box-9a and Nuclear receptor subfamily 5 group number 1b were significantly decreased by 25 ng/L, but not 5 ng/L EE2 compared with the levels in untreated fish. The decreased transcription was correlated with a previously shown spawning failure in these males (Reyhanian et al., 2011. Aquat Toxicol 105, 41-48), suggesting that decreased mRNA expression of genes regulating male sexual function could be involved in the functional sterility. The mRNA level of Cytochrome P-45019a, involved in female reproductive development, was unaffected by hormone treatment. The transcription of the female-specific Vitellogenin was significantly induced in testes. While testicular Androgen Receptor and the Estrogen Receptor-alpha mRNA levels were unchanged, Estrogen receptor-beta was significantly decreased by 25 ng/L EE2. Hepatic Estrogen Receptor-alpha mRNA was significantly increased by both exposure concentrations, while Estrogen Receptor-beta transcription was unaltered. The decreased transcription of male-predominant genes supports a demasculinization of testes by EE2 and might reflect reproductive disturbances in the environment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 164, August 2014, Pages 35-42
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 164, August 2014, Pages 35-42
نویسندگان
Nasim Reyhanian Caspillo, Kristina Volkova, Stefan Hallgren, Per-Erik Olsson, Inger Porsch-Hällström,