کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8319878 | 1539347 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recent advances in engineering microbial rhodopsins for optogenetics
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Recent advances in engineering microbial rhodopsins for optogenetics Recent advances in engineering microbial rhodopsins for optogenetics](/preview/png/8319878.png)
چکیده انگلیسی
Protein engineering of microbial rhodopsins has been successful in generating variants with improved properties for applications in optogenetics. Members of this membrane protein family can act as both actuators and sensors of neuronal activity. Chimeragenesis, structure-guided mutagenesis, and directed evolution have proven effective strategies for tuning absorption wavelength, altering ion specificity and increasing fluorescence. These approaches facilitate the development of useful optogenetic tools and, in some cases, have yielded insights into rhodopsin structure-function relationships.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Structural Biology - Volume 33, August 2015, Pages 8-15
Journal: Current Opinion in Structural Biology - Volume 33, August 2015, Pages 8-15
نویسندگان
R Scott McIsaac, Claire N Bedbrook, Frances H Arnold,