Sensitive probes of protein structure and dynamics in well-controlled environments: combining mass spectrometry with fluorescence spectroscopy

- Fluorescence is a recent addition to the mass spectrometry-enabled technical toolbox.
- Selectivity provided by MS enables dissection of complex intermolecular interactions.
- FRET of mass-selected de-solvated biomolecules provides nm-range distance constraints.
- Dynamic quenching measurements assess conformational fluctuations.
- These tools provide insight into intrinsic protein structure, stability and dynamics.