Single-molecule FRET and crosslinking studies in structural biology enabled by noncanonical amino acids

- Genetic code expansion technology has significantly influenced structural biology.
- Crosslinking through noncanonical amino acids (ncAAs) has been used to build 3D models.
- High-resolution single-molecule FRET experiments are facilitated by incorporation of ncAAs.
- Whole-genome-recoded organisms can become ideal expression hosts for use by structural biologists.