کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8320470 | 1539384 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Insights into the complex levels of regulation imposed on Escherichia coli DNA polymerase V
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
TLSdNTPrNTPY-family DNA polymerasecpd - CPDdeoxyribonucleoside triphosphate - دز اکسید ریبونوکلئوزید تری فسفاتcyclobutane pyrimidine dimer - دییریر پیریمیدین cyclobutaneDNA polymerase - دیانای پلی مراز، DNA پلیمرازribonucleoside triphosphate - ریبونوکلئوزید تری فسفاتtranslesion DNA synthesis - سنتز DNA ترجمه شدهPosttranslational regulation - مقررات پستیNucleotide - نوکلئوتیدProteolysis - پروتئولیزpol - پل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It is now close to 40 years since the isolation of non-mutable umu/uvm strains of Escherichia coli and the realization that damage induced mutagenesis in E.coli is not a passive process. Early models of mutagenesis envisioned the Umu proteins as accessory factors to the cell's replicase that not only reduced its normally high fidelity, but also allowed the enzyme to traverse otherwise replication-blocking lesions in the genome. However, these models underwent a radical revision approximately 15 years ago, with the discovery that the Umu proteins actually encode for a DNA polymerase, E.coli pol V. The polymerase lacks 3â²Â â 5â² exonucleolytic proofreading activity and is inherently error-prone when replicating both undamaged and damage DNA. So as to limit any “gratuitous” mutagenesis, the activity of pol V is strictly regulated in the cell at multiple levels. This review will summarize our current understanding of the myriad levels of regulation imposed on pol V including transcriptional control, posttranslational modification, targeted proteolysis, activation of the catalytic activity of pol V through protein-protein interactions and the very recently described intracellular spatial regulation of pol V. Remarkably, despite the multiple levels at which pol V is regulated, the enzyme is nevertheless able to contribute to the genetic diversity and evolutionary fitness of E.coli.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 44, August 2016, Pages 42-50
Journal: DNA Repair - Volume 44, August 2016, Pages 42-50
نویسندگان
Myron F. Goodman, John P. McDonald, Malgorzata M. Jaszczur, Roger Woodgate,