In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C

- Knock-in mice that express catalytically-inactive Polk have been established.
- Mutations at CpG and GpG sites where MMC forms cross-links were enhanced in the mice.
- DNA strand breaks induced by MMC were also enhanced in the mice.
- Polk mediates error-free bypass across MMC cross-links and averts DNA strand breaks.