کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8320745 | 1539405 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NAD derived second messengers: Role in spontaneous diastolic Ca2+ transients in murine cardiac myocytes
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کلمات کلیدی
RyRNAADPADP-ribosyl cyclaseScT - ScT در[Ca2+]i - [Ca2 +] inicotinic acid adenine dinucleotide phosphate - اسید نیکوتین اسید آدنین دینکلوتید فسفاتISO - ایزوisoproterenol - ایزوپروترنولSarcoplasmic reticulum - رتیکولوم سارکوپلاسمیکCalcium signaling - سیگنالینگ کلسیمSERCA - قلبCardiac myocyte - میوسیت قلبیSignal transduction - هدایت سیگنالRyanodine receptor - گیرنده رایانودین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: NAD derived second messengers: Role in spontaneous diastolic Ca2+ transients in murine cardiac myocytes NAD derived second messengers: Role in spontaneous diastolic Ca2+ transients in murine cardiac myocytes](/preview/png/8320745.png)
چکیده انگلیسی
Strong β-adrenergic stimulation induced spontaneous diastolic Ca2+ transients (SCTs) in electrically paced murine cardiac myocytes [28]. To obtain further insights into the underlying mechanism, we developed a method for a simultaneous analysis, in which the free luminal Ca2+ concentration in the sarcoplasmic reticulum (SR) ([Ca2+]SR) and the free cytosolic Ca2+ concentration ([Ca2+]i) were measured in parallel in the same cell. Each spontaneous diastolic Ca2+ transient was exactly mirrored by a decrease of [Ca2+]SR. Since antagonism of the Ca2+ mobilizing second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) was shown to block SCTs in single cardiac myocytes [28], we analyzed the effect of the novel ADP-ribosyl cyclase inhibitor SAN4825 on both cytosolic and intra-luminal Ca2+ transients upon strong β-adrenergic stimulation. A strong antagonist effect of SAN4825 on SCTs at low micromolar concentrations was observed. Our results suggest that the underlying mechanism of spontaneous diastolic Ca2+ transients observed upon strong β-adrenergic stimulation is sensitization of type 2 ryanodine receptor by the Ca2+ releasing activity of the products of ADP-ribosyl cyclase activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 23, November 2014, Pages 69-78
Journal: DNA Repair - Volume 23, November 2014, Pages 69-78
نویسندگان
Dominik Warszta, Merle Nebel, Ralf Fliegert, Andreas H. Guse,