کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8323950 | 1539900 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
FoxO regulates expression of ABCA6, an intracellular ATP-binding-cassette transporter responsive to cholesterol
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کلمات کلیدی
PI3KFITCDTTTRITCGAPDHIGF-1insulin-like growth factor-1 - انسولین مانند عامل رشد 1Cho - برایtetramethylrhodamine isothiocyanate - تترامتییل رودامین ایزوتیوسیاناتChinese Hamster Ovary - تخمدان هامستر چینیABC transporters - حمل کننده ABCdithiothreitol - دیتیوتریتولEndothelial cells - سلولهای اندوتلیالPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتforkhead box protein - پروتئین جعبه جعبهcholesterol - کلسترولglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
ATP-binding-cassette (ABC) proteins have been recognized as key players in cellular physiological transport processes. ABC transporter A6 (ABCA6) is a member of the ABC subfamily A. Although it was cloned more than 10 years ago, its expression regulation, subcellular localization, and physiologic function remain largely unknown. We here demonstrated that expression of ABCA6 was Forkhead box O (FoxO)-dependent in human endothelial cell line EA.hy926 and human umbilical vein endothelial cells. Two functional FoxO-responsive elements were identified in ABCA6 promoter and characterized in detail. ABCA6 mRNA was suppressed by insulin-like growth factor-1 which stimulates the phosphorylation and inactivation of FoxOs while inhibitor of phosphatidylinositol 3-kinase had the opposite effect. By immunofluorescence and confocal microscopy, ABCA6 protein is localized primarily in an intracellular compartment, likely representing the Golgi apparatus. ABCA6 mRNA was demonstrated to be responsive to cholesterol loading as well as 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase inhibitors in human endothelial cells. Our data provide evidence for an essential role of FoxO proteins in the transcription of ABCA6 in human vascular endothelial cells. Based on its cholesterol responsiveness, a potential involvement of ABCA6 in intracellular lipid transport processes may be anticipated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 11, November 2013, Pages 2651-2659
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 11, November 2013, Pages 2651-2659
نویسندگان
Junfang Gai, Meiling Ji, Chenxi Shi, Wenli Li, Simin Chen, Yeyu Wang, Hao Li,