Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression

► We compared Sema3A expression in satellite cell cultures from EDL/soleus muscles. ► Sema3A expression was higher in soleus muscle satellite cells. ► Recombinant Sema3A treatment stimulated myogenin expression in satellite cells. ► Sema3A receptor plexin A2 expression was higher in soleus muscle satellite cells. ► The comparative study highlights a possible Sema3A-plexin A2-myogenin signaling axis.