کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8324958 | 1539929 | 2011 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The transmembrane domain of podoplanin is required for its association with lipid rafts and the induction of epithelial-mesenchymal transition
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کلمات کلیدی
CTBDRME-cadherin - E-CadherinENaC - ENACEMT - تکنسین فوریتهای پزشکیextracellular - خارج سلولیcytoplasmic - سیتوپلاسمیکdetergent-resistant membrane - غشای مقاوم در برابر مواد شویندهmethyl-β-cyclodextrin - متیل-β-سیکلوکودکسترینcaveolin-1 - کائولین-1Cav-1 - کاو-1cholera toxin B-subunit - کلسترول توکسین B-subunitepithelial-to-mesenchymal transition - گذار اپیتلیال به مزانشیمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Podoplanin is a transmembrane glycoprotein that is upregulated in cancer and was reported to induce an epithelial-mesenchymal transition (EMT) in MDCK cells. The promotion of EMT was dependent on podoplanin binding to ERM (ezrin, radixin, moesin) proteins through its cytoplasmic (CT) domain, which led to RhoA-associated kinase (ROCK)-dependent ERM phosphorylation. Using detergent-resistant membrane (DRM) assays, as well as transmembrane (TM) interactions and ganglioside GM1 binding, we present evidence supporting the localization of podoplanin in raft platforms important for cell signalling. Podoplanin mutant constructs harbouring a heterologous TM region or lacking the CT tail were unable to associate with DRMs, stimulate ERM phosphorylation and promote EMT or cell migration. Similar effects were observed upon disruption of a GXXXG motif within the TM domain, which is involved in podoplanin self-assembly. In contrast, deletion of the extracellular (EC) domain did not affect podoplanin DRM association. Together, these data suggest that both the CT and TM domains are required for podoplanin localization in raft platforms, and that this association appears to be necessary for podoplanin-mediated EMT and cell migration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 43, Issue 6, June 2011, Pages 886-896
Journal: The International Journal of Biochemistry & Cell Biology - Volume 43, Issue 6, June 2011, Pages 886-896
نویسندگان
Beatriz Fernández-Muñoz, MarÃa M. Yurrita, Ester MartÃn-Villar, Patricia Carrasco-RamÃrez, Diego MegÃas, Jaime Renart, Miguel Quintanilla,