کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8326270 | 1539965 | 2008 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ginkgolides mimic the effects of hypoxic preconditioning to protect C6 cells against ischemic injury by up-regulation of hypoxia-inducible factor-1 alpha and erythropoietin
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کلمات کلیدی
PI3Kpharmacological preconditioningp-ERKEGb 761TNF-alphaEPOOGDHIF-1αN-methyl-d-aspartateNMDAMAPK - MAPKHypoxic preconditioning - preconditioning hyppoxicAkt - آکتerythropoietin - اریتروپویتینtumor necrosis factor-alpha - تومور نکروز عامل آلفاDOR - دردserine-threonine protein kinase - سرین ترئونین پروتئین کینازVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)phosphatidylinositol 3′-kinase - فسفاتیدیلینواستیل 3'-kinaseNeuroprotection - محافظت نورونی یا محافظت از عصبoxygen–glucose deprivation - محرومیت از اکسیژن-گلوکزhypoxia-inducible factor-1 alpha - هیپوکسی القاء عامل 1 آلفاmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenphosphorylated extracellular signal-regulated kinase - کیناز تنظیم شده سیگنال تنظیم شده خارج سلولی فسفورید شدهdelta-opioid receptor - گیرنده دلتا-اپیوئیدGinkgolides - گینکولیدس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Hypoxic preconditioning can play a significant neuroprotective role. However, it has not been employed clinically because of safety concerns. To find a safer preconditioning stimulus that is both practical and effective, we investigated whether ginkgolides are capable of preconditioning as hypoxia to protect C6 cells against ischemic injury. We demonstrated that both ginkgolides (37.5 μg/mL) and hypoxia (1% O2 for 16 h) can significantly increase cell viabilities and expression of phosphorylated glycogen synthase kinase (p-GSK), phosphorylated extracellular signal-regulated kinase (p-ERK), hypoxia-inducible factor-1 alpha (HIF-1α) and erythropoietin (EPO) in ischemic cells. The inhibitors of mitogen-activated protein kinase (MAPK) or phosphatidylinositol 3â²-kinase (PI3K) significantly but not completely reduced the enhanced expression of these proteins and cell viabilities induced by ginkgolides and hypoxic preconditioning. These indicated that ginkgolides could mimic hypoxic preconditioning by increasing expression of HIF-1α as well as its target protein EPO and that the ginkgolides and hypoxic preconditioning role might be partly mediated by the activation of the p42/p44-mitogen-activated protein kinase and phosphatidylinositol 3â²-kinase/AKT/glycogen synthase kinase 3beta pathways. The similar tendency in the changes of protein expression, cell viabilities and responses to MAPK or PI3K inhibitors of the cells treated with ginkgolides and hypoxia suggests that ginkgolides and hypoxic preconditioning might operate by similar mechanisms. The findings also imply that ginkgolides might have the potential for clinical use to prevent injury in high-risk conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 40, Issue 4, 2008, Pages 651-662
Journal: The International Journal of Biochemistry & Cell Biology - Volume 40, Issue 4, 2008, Pages 651-662
نویسندگان
He Wei, Qian Zhong Ming, Zhu Li, Christopher Qian, Du Fang, Yung Wing Ho, Ke Ya,