Interfacial and emulsion stabilized behavior of lysozyme/xanthan gum nanoparticles

Interfacial adsorption kinetics and stabilizing emulsion behavior of lysozyme/xanthan gum nanoparticles (Ly/XG NPs) by variation of particle size and energy input. The interfacial rheology indicated that interfacial adsorption behavior of Ly/XG NPs displayed in a particle size manner. Increasing the particle size of Ly/XG NPs hindered its initial diffusion onto the interface. Smaller size was helpful for its fast diffusion to the interface. KP (rate constant of penetration) and KR (rate constant of penetration) of Ly/XG NPs were both affected by particle size. The KP for adsorbed Ly/XG NPs increased as the particle sizes increased. KR was considerably higher than KP, indicating the structural rearrangement of adsorbed Ly/XG NPs played an important part in interfacial film formation. The morphology of Pickering emulsion indicated its drop sizes were determined by the oil/aqueous volume fraction and prepared style. The higher energy input the size become smaller. Based on interfacial adsorption kinetics and microstructure of Pickering emulsions, the stabilization behavior was related to particle-particle associations and conformational changes of Ly/XG NPs. This work confirmed Ly/XG NPs could form Pickering emulsion by selecting different particle size and emulsification process, and offer promising prospects in stabilizing emulsion with the demands of surfactant-free.