کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8327116 1540198 2018 28 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protective and antioxidative effect of rubropunctatin against oxidative protein damage induced by metal catalyzed reaction
ترجمه فارسی عنوان
اثر محافظتی و آنتی اکسیدانی روبروپونکتاتین در برابر آسیب پروتئین اکسیداتیو ناشی از واکنش کاتالیزوری فلزی
کلمات کلیدی
روبروپونکتاتین، رنگدانه، آنتی اکسیدان، اکسیداسیون پروتئین، کربنایل،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Monascus purpureus is known to produce several coloured secondary metabolites. In this study, M. purpureus CFR 410-11 mutant fermented with rice was dried and extracted in hexane for purification of pigment. The purity of the isolated pigment was confirmed by different chromatography techniques. The spectroscopic analysis revealed its structural identity as rubropunctatin. The antioxidant potencies of isolated rubropunctatin were evaluated. Rubropunctatin scavenged 16% 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and inhibited 20% superoxide generation at 8 μg/ml concentration. The multiple antioxidant abilities of rubropunctatin were evidenced by its ferric reducing capacity also. The oxidative damage of BSA protein was induced by the metal catalyzed oxidation (MCO) by Fe2+/H2O2. The protective effects of rubropunctatin and M. purpureus (MTCC-410 and CFR 410-11) extracts were compared with glutathione and ascorbic acid. The M. purpureus extracts and rubropunctatin inhibited the formation of carbonyl content and protein oxidation assayed by SDS-PAGE. Rubropunctatin (42-169 μM) efficiently inhibited the protein oxidation compared to glutathione (48-195 μM) and ascorbic acid (85-340 μM) by scavenging the superoxide and hydroxyl radical generated in the system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 116, September 2018, Pages 409-416
نویسندگان
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