کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8327207 | 1540200 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and biochemical characterization of α-ketoglutarate decarboxylase from cyanobacterium Synechococcus sp. PCC7002
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کلمات کلیدی
HEPESNADPTRISN-(2-Hydroxyethyl)piperazine-N′-2-ethanesulfonic acid - N- (2-Hydroxyethyl) piperazine-N'-2-ethanesulfonic acidsodium dodecyl sulphate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدProtein expression - بیان پروتئینBis-tris - بیس-تریسTris(hydroxymethyl)aminomethane - تریس (هیدروکسی متیل) آمینومتانCatalytic function - عملکرد کاتالیزوریKinetic characterization - مشخصه جنبشیnicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphate
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
α-Ketoglutarate decarboxylase (α-KGD), one member of α-keto acid decarboxylases, catalyzing non-oxidative decarboxylation of α-ketoglutarate to form succinic semialdehyde, was proposed to play critical role in completing tricarboxylic acid (TCA) cycle of cyanobacteria. Although the catalytic function of α-KGD from Synechococcus sp. PCC7002 was demonstrated previously, there was no detailed biochemical characterization of α-KGD from Synechococcus sp. PCC7002 yet. In this study, the gene encoding α-KGD from Synechococcus sp. PCC7002 was amplified and soluble expression of recombinant α-KGD was achieved by coexpressing with pTf16 chaperone plasmid in E. coli BL21 (DE3). Kinetic analysis showed that the activity of α-KGD was dependent on cofactors of thiamine pyrophosphate and divalent cation. Meanwhile this α-KGD was specific for α-ketoglutarate with respect to the decarboxylation activity despite of the pretty low activity of acetolactate synthase. The catalytic efficiency of α-KGD (the values of kcat and kcat/Km for α-ketoglutarate were 1.2 sâ1 and 6.3 Ã 103 Mâ1 sâ1, respectively) might provide evidence for its physiological role in TCA cycle of Synechococcus sp. PCC7002.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 114, 15 July 2018, Pages 188-193
Journal: International Journal of Biological Macromolecules - Volume 114, 15 July 2018, Pages 188-193
نویسندگان
Guofeng Lei, Xiaoqin Wang, Chongde Lai, Zhi-Min Li, Wei Zhang, Congcong Xie, Fei Wang, Xiaoyu Wu, Zhimin Li,