کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8327440 | 1540199 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polysaccharides from Diaphragma juglandis fructus: Extraction optimization, antitumor, and immune-enhancement effects
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
This study discusses the optimization of the microwave-assisted extraction of polysaccharides from Diaphragma juglandis (DJPs). One main fraction (DJP-2) was successfully purified by ion-exchange chromatography and gel-permeation chromatography. The results showed that the optimal extraction conditions with DJP extraction yield of 4.7â¯Â±â¯0.28% were water to raw material ratio of 20â¯mL/g, extraction time of 40â¯min, and microwave extraction power of 400â¯W. Bioassays indicated that DJP-2 could effectively suppress the proliferation of HepG2 and BGC-82 cell lines. In addition, DJP-2 could significantly enhance phagocytosis; stimulate the production of NO, tumor necrosis factor-α (TNF-α), and interleukins (IL-6 and IL-1β); and promote their corresponding mRNA expression levels in a dose-dependent manner. Meanwhile, CR3, MR, and TLR2 were confirmed to be the major membrane receptors of DJP-2 on RAW 264.7. All these results indicated that DJP-2 could be a potential antitumor and immunomodulatory agent in the field of pharmacology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 115, August 2018, Pages 835-845
Journal: International Journal of Biological Macromolecules - Volume 115, August 2018, Pages 835-845
نویسندگان
Qingran Meng, Yaqi Wang, Feng Chen, Tiancun Xiao, Lianfu Zhang,